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2023, № 107

UDC: 619:636.5.085.12
GSNTI: 68.41.35

Chelating feed additive effect on rat gut microbiome

The article presents the results of studying the effect of the cobalt chelate compound in vitro and in vivo on the useful microflora and the opportunistic pathogenic group microorganisms. The qualitative and quantitative composition of the rats gut microbiome under the influence of a chelate feed additive developed for use in animal husbandry has been studied. It has been found that under in vitro conditions, the compound under study showed no inhibitory effect on the microorganisms reference strains. Subsequently, in vivo, two groups of rats treated with 153.0 mg/kg and 307.0 mg/kg live weight were shown to have a stimulating effect of the feed additive on representatives of the rats beneficial colon microflora. It was found that a feed supplement at a dose of 153.0 mg/kg of live weight stimulates the development of beneficial microflora in the large intestine of rats, and at a dose of 307.0 mg/kg, on the contrary, significantly restrains its development. In this case, the gut microbiome of the control group rats that did not receive the supplement included not only a higher number of CPB, but also a wider range of the cocci bacteria compared to rats of the experimental groups. Thus, the amount of Ligolactobacillus murinus in the rats gut microbiome of the first test group was significantly higher by 1.4 times compared to the control group (p ≤ 0.05) and 1.9 times higher than in rats of the second test group. Chelated feed additive has been found to improve gut microecology by increasing the proportion of useful microflora populations and reducing the number of opportunistic bacteria.
Keywords: Feed additive, chelate compound, cobalt, methionine, gut microbiome, quantitative composition, species identification, time-of-flight mass spectrometry.
DOI: 10.21515/1999-1703-107-193-198

References:

1. Горковенко, Н. Е. Влияние функциональной кормовой добавки на продуктивность, иммунный статус и микробиом кишечника кур-несушек / Н. Е. Горковенко, Н. Н. Бондаренко, А. Н. Шевченко, Д. О. Алферов // Труды Кубанского государственного аграрного университета. - 2023. - № 104. - С. 139-146.
2. Горковенко, Н. Е. Количественная оценка кишечного микробиоценоза телят / Н. Е. Горковенко, Ю. А. Макаров, А. М. Кузьменко. - Труды ВИЭВ. - 2009. - Т. 75. - С. 176-178.
3. Горковенко, Н. Е. Вариабельность ассоциаций микроорганизмов, этиологически значимых в инфекционной патологии животных / Н. Е. Горковенко, К. Н. Таранова, О. В. Бородинова. - Год науки и технологий 2021: сб. тез. по материалам Всерос. науч.-прак. конф. - Краснодар, 2021. - C. 40.
4. Макаров, Ю. А. Применение цеолитов для снижения отрицательного влияния экологических факторов на организм бройлеров / Ю. А. Макаров, Н. Е. Горковенко // Дальневосточный аграрный вестник, 2010. - № 4(16). - С. 29-31.
5. Методические рекомендации MP 4.2.0089-14. Использование метода времяпролетной масс-спектрометрии с матрично-активированной лазерной десорбцией/ионизацией (MALDI-ToF MS) для индикации и идентификации возбудителей I-II групп патогенности. - М.; 2014. - https://www.mediasphera.ru/issues/laboratornaya-sluzhba/2018/2/12305219820 18021078.
6. Новикова, О. Кормовые добавки для профилактики бактериальных болезней в птицеводстве / O. Новикова, A. Сафонов // Эффективное животноводство. - 2019. - № 4(152). - C. 57-60.
7. Макаров, Ю. А. Особенности дальневосточных природных изолятов Bacillus thuringiensis / Ю. А. Макаров, Н. Е. Горковенко, В. А. Серебрякова, Т. С. Малоквасова. - Труды Всероссийского НИИ экспериментальной ветеринарии им. Я. Р. Коваленко. - 2009. - Т. 75. - С. 446-448.
8. Velazquez-Meza, M. E. Antimicrobial resistance: One Health approach / M. E. Velazquez-Meza, M. Galarde-López, B. Carrillo-Quiróz, C. M. Alpuche-Aranda // Veterinary World. - 2022. - No. 15(3). - P. 743-749.
9. Bharathi, S. Functional Feed Additives Used in Fish Feeds / S. Bharathi, C. Antony, C. Rajagopalasamy // Int. J. Fish. Aquat. Stud. 2019. - V. 7. - P. 44-52.
10. Hooper, L. V.Interactions between the Microbiota and the Immune System / L. V. Hooper, D. R. Littman, A. J. Macpherson // Science. - 2012. - V. 336. - P. 1268-1273. - DOI: 10.1126/science.1223490.
11. Ogunkalu, O. Effects of Feed Additives in Fish Feed for Improvement of Aquaculture / O. Ogunkalu // Eurasian J. Food Sci. Technol. - 2019. - V. 3. - P. 49-57.
12. Reda, R. M. Evaluation of Bacillus amylo-liquefaciens on the Growth Performance, Intestinal Morphology, Hematology and Body Composition of Nile tilapia, Oreochromis niloticus / R. M. Reda, K.M. Selim // Aquac.Int. 2014. - V. 23. - P. 203-217. - DOI: 10.1007/s10499-014-9809-z.
13. Sarno, E. A Review of Significant European Foodborne Outbreaks in the Last Decade / E. Sarno, D. Pezzutto, M. Rossi, E. Liebana, V. Rizzi // J Food Prot. - 2021. - Dec 1; 84(12):2059-2070. - Doi: 10.4315/JFP-21-096. PMID: 34197583.
14. Steiner, T. Phytogenics in Animal Nutrition: Natural Concepts to Optimize Gut Health and Performance. Nottingham University Press; Nottingham, UK: 2009.
15. Walters, M. Potential of Food Hydrolyzed Proteins and Peptides to Chelate Iron or Calcium and Enhance Their Absorption / M. Walters, R. Esfandi, A. Tsopmo // Foods. - 2018. - V. 7. - P. 172. - DOI: 10.3390/foods7100172.

Authors:

1. Gorkovenko Natalya Evgenievna, DSc in Biology, professor, Federal State Budgetary Educational Institution of Higher Education "I.T. Trubilin Kuban State Agrarian University".
2. Kopyltsov Sergey Vasilievich, PhD in Biology, Federal State Budgetary Educational Institution of Higher Education "I.T. Trubilin Kuban State Agrarian University".
3. Kosykh Anastasia Valerievna, graduate student, Federal State Budgetary Educational Institution of Higher Education "I.T. Trubilin Kuban State Agrarian University".