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2023, № 106

UDC: 633.18.03
GSNTI: 68.35.03, 68.35.29

Phenotyping of experimental rice population in accelerated breeding of new varieties with valuable grain quality characteristics

The study of the consumer advantages of rice samples was carried out in connection with the development of an effective breeding methodology for creating rice genotypes based on an accelerated breeding process. The aim of the study was to study the protein and amylose content in grain samples of parent forms and BC2 samples. In the program of creating varieties based on the use of modern postgenomic and cellular technologies, BC2 and their parent forms - samples of the UNU "Collection of the FNC rice" belonging to various groups in the form of grain, white-grained, served as the research material. The crop is grown on the OPU FNC rice. As part of a scientific study, samples with an unpainted pericarp of a grain were isolated for large-scale genotyping. Phenotyping of the studied rice samples was carried out according to the content of protein and amylose in rice grains. The protein content was evaluated using a Universal Infrared express analyzer MultiCheck Plus", Infra Lum FT 6, the amylose content in starch - according to GOST ISO 6647-2-2015 Fig. Determination of amylose content. Part 2. BC2 groups with a high protein content, more than 8.8% and amylose content were identified in BC2 grain combinations 1 - 16,0-19,0 %, 2 - amylose content 20.0-22.0%, 3 - amylose content 22.1-27.0%.
Keywords: Rice, variety, nutritional advantages, protein content, amylose content, backcrosses, rice breeding.
DOI: 10.21515/1999-1703-106-278-283

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Authors:

1. Mukhina Zhanna Mikhaylovna, DSc in Biology, Federal State Budgetary Scientific Institution "Federal Research Center of Rice".
2. Tumanyan Natalya Georgievna, DSc in Biology, professor, Federal State Budgetary Scientific Institution "Federal Research Center of Rice".
3. Papulova Elina Yurievna, PhD in Biology, Federal State Budgetary Scientific Institution "Federal Research Center of Rice".
4. Yesaulova Lyubov Vladimirovna, PhD in Biology, Federal State Budgetary Scientific Institution "Federal Research Center of Rice".
5. Garkusha Sergey Valentinovich, DSc in Agriculture, Corresponding member RAS, Federal State Budgetary Scientific Institution "Federal Research Center of Rice".