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2022, № 102

UDC: 619:636.5:577.18:615.281.9
GSNTI: 68.41.31,68.41.35

Antimicrobial resistance in dairy farming and food products

We studied the distribution of conditionally pathogenic and pathogenic microorganisms on livestock objects, milk and their sensitivity to nine groups of antimicrobials. It was found that in all samples of wipes from contact surfaces, dairy farm equipment, milk, as well as skin, mucous membranes, cervical and nasal secretions of cows and young animals, the same type of microorganisms of pathogenic and conditionally pathogenic flora were identified. Milk samples also revealed representatives of pathogenic and conditionally pathogenic microflora: enterococci (E. faecium and E. faecalis), golden staphylococcus (S. aureus), E. coli (E. Coli) hemolyzing. The microflora structure revealed an excessive number of pathogens, primarily Staphylococcus aureus, and a low number of saprophytic staphylococci. The results of determining the sensitivity of the microflora to nine antibiotics showed that 44% of strains showed low sensitivity or complete resistance to AMF. Genetic study revealed that among the detected members of the family Enterobacteriaceae the frequency of detection of E. coli and Enterobacter spp. isolates possessing mechanisms for transmissible transmission of gene material was the highest. Mutation genes responsible for antimicrobial resistance were detected in 53% of E. coli.
Keywords: Antimicrobials, resistant microorganisms, antibiotic susceptibility, dairy farms.
DOI: 10.21515/1999-1703-102-275-280

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Authors:

1. Moiseeva Ksenia Viktorovna, researcher, FGBNU UrFARC UB RAS.
2. Loginov Egor Alexandrovich, PhD student, FGBNU UrFARC UB RAS.
3. Zubareva Vladlena Dmitrievna, PhD student, FGBNU UrFARC UB RAS.
4. Krivonogova Anna Sergeevna, DSc in Biology, leading scientific employee, FGBNU UrFARC UB RAS.
5. Isaeva Albina Gennadievna, DSc in Biology, leading researcher, FGBNU UrFARC UB RAS.
6. Sokolova Olga Vasilievna, DSc in Medical, leading researcher, FGBNU UrFARC UB RAS.
7. Donnik Irina Mikhailovna, DSc in Biology, academician of the RAS, Ch. researcher, FGBNU UrFARC UB RAS.