<<<  Back

2023, № 106

UDC: 57.085.23+577.21
GSNTI: 68.35.03,62.33.29

Improvement of methods for accelerated production of experimental populations of rice L

Some factors have been studied in the development of an effective protocol for optimizing technologies for obtaining seedlings from hybrid embryos and culture of rice anthers in vitro. It is shown that the release of the program for the automatic development of conception in vitro is determined by the sequence of its development, the type of family man and the composition of the nutrient medium, for the realization of someone's superiority and cultivation. Ionizing radiation of callus in the culture of anthers was produced on the basis of N6 and Blades supplemented with 2,4-D and BAP. A well-known study showed that the combination of hormones 2,4-D (2.0 mg/l) and BAP (0.5 mg/L) in the composition of the Blinds was more effective, it enhanced the induction of the callus name not only in the hybrid Japanese/japonica and japonica japonica/indika, but also in the hybrid indica/indika.
Keywords: Orysa sativa L, in vitro anther culture, induction medium, regenerant, DH-lines.
DOI: 10.21515/1999-1703-106-329-336

References:

1. Бутенко, Р. Г. Культура изолированных клеток и тканей в селекции растений / Р. Г. Бутенко, Г. И. Тихонович [и др.]. - Основы сельскохозяйственной биотехнологии, 1990. - С. 162-165.
2. Dwivedi, S. L. Haploids: Constraints and opportunities in plant breeding / S. L. Dwivedi, A. B. Britt, L. Tripathi, S. Sharma, H. D. Upadhyay, R. Ortiz / Biotechnology Advances, 1 November 2015. - Vol.33. - Issue 6. - Part 1. - P. 812-829. - DOI: https://doi.org/10.1016/j.biotechadv.2015.07.001.
3. Afza, R. Effect of spikelet position on rice anther culture efficiency / R. Afza, M. Shen, F. J. Zapata-Arias, J. Xie, H.K. Fundi, K.S. Lee et al. / Plant Sci., 2000. - Vol.153(2). - Р. 155-159. - DOI: https://doi.org/10.1016/s0168-9452(99)00266-6 PMID: 10717321.
4. Naik, N. Development of doubled haploids from an elite indica rice hybrid (BS6444G) using anther culture / N. Naik, P. Rout, U. Ngangkham, R. L. Verma, J. L. Katara, K. K. Sahoo et al. // Plant Cell Tissue Organ Cult. - 2017. - Vol. 128(3) - Р. 679-689.
5. Yi, G. H. Effects of cold shock pretreatment and carbohydrate sources on anther culture of rice / G. H. Yi, Y. J. Won, J. M. Ko, H. M. Park, J. H. Cho, B. G. et al. //j. Plant Biotechnol. - 2003. - Vol.30(4). - Р. 369-373.
6. Mayakaduwa, D. M. Anther culture as a supplementary tool for rice breeding / D. M. Mayakaduwa, T. D. Silva / In: Shah F, Khan ZH, Iqbal A editors. Rice Crop-Current Developments. - 2018. - Vol.5. - Р. 1-5.
7. Wedzony, M. Progress in doubled haploid technology in higher plants / M. Wedzony, B. P. Foster, I. Zur, E. Golemiec, M. Szechynska-Hebda, E. Dubas, G. Gotebiowska // Advanced in haploid production in higher plants. - Springer Science + Business Media B.V., 2009. - Р. 1-35.
8. Grewal, D. Doubled haploids generated through anther culture from crosses of elite indica and japonica cultivars and/or lines of rice: large scale production, agronomic performance and molecular characterization / D. Grewal, C. H. Manito and V. Bartolome / Crop Sci. - 2011. - Vol. 51(6). - Р. 2544-2553.
9. Dewi, I. S. Plant regeneration from anther cultures of several genotypes of indica rice tolerant to aluminum toxicity / I. S. Dewi, B. S. Purwoko, H. Aswidinnoor, I. H. Somantri, M. A. Chozin // Indonesian J. Agric. - 2009. - Vol. 2(1). - Р. 1-5.
10. Ali, J. Improved anther culture media for enhanced callus formation and plant regeneration in rice (Oryza sativa L.) /j. Ali, K. L. Nicolas, S. Akther, A. Torabi, A. A. Ebadi, C. M. Marfori-Nazarea et al. // Plants. - 2021. - Vol. 10 (5). - Р. 839. - DOI: https://doi.org/10.3390/plants10050839 PMID: 33921954.
11. Dipti, V. Protocol for in vitro somatic embryogenesis and regeneration of rice (Oryza sativa L.) / V. Dipti, J. Rohit, A. Shukla, K. Pramod // Indian Journal of Experimental Biology. - 2011. - Vol. 49(12). - Р. 958-63.
12. Parisa, A.Comptes Rendus Highly efficient protocol for callogenesis, somagenesis and regeneration of Indica / A. Parisa, Y.R. Mohd, M. Maziah, M. H. Mohamed, A. A. Siti Nor, A. Rambod, S. Mahbod // Biologies. - 2015. - Vol. 338. - Issue 7. - P. 463-470. - DOI: https://doi.org/10.1016/j.crvi.2015.04.004.
13. Blaydes, D. F.Interaction of kinetin and various inhibitors in the growth of soybean tissue // Physiol Plant. - 1966. - Vol. 19. - Р. 748-753.
14. Chu, C. C. The N6 medium and its applications to anther culture of cereal crops / C. C. Chu // In Proceedings of Symposium on Plant Tissue Culture. Science Press. - 1978. - Vol. 43. - Р. 50.

Authors:

1. Savenko Elena Georgievna, PhD in Biology, senior researcher, Federal State Funded Scientific Institution “Federal Scientific Rice Center”.
2. Mukhina Zhanna Mikhailovna, DSc in Biology, leading researcher, Federal State Funded Scientific Institution “Federal Scientific Rice Center”.
3. Glazyrina Valentina Aleksandrovna, senior researcher, Federal State Funded Scientific Institution “Federal Scientific Rice Center”.
4. Korotenko Tatyana Leonidovna, PhD in Agriculture, senior researcher, Federal State Funded Scientific Institution “Federal Scientific Rice Center”.
5. Shundrina Lyudmila Anatolevna, researcher, Federal State Funded Scientific Institution “Federal Scientific Rice Center”.