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2023, № 106

UDC: 633.11:632.938.1+575.117
GSNTI: 68.37.13

Surfactin of bacterial strain 26D induce systemic resistance of wheat plants against greenbug aphid (Rond.)

Greenbug aphid (Schizaphis graminum Rond.) causes significant damage to wheat, barley, rye, oats and rice. The use of endophytic bacteria and their metabolites can improve the protection of crops from the pest. Lipopeptides are one of the metabolites of Bacillus bacteria of particular scientific interest. In our laboratory, a bacterial line B. subtilis 26Dsfp- deficient in surfactin synthesis with an inactive 4-phosphopanthenyl transferase (sfp) gene was obtained. This made it possible to study the effect of surfactin on the induction of defense reactions in wheat plants against greenbug aphids. The effect of surfactin on the growth promoting and insecticidal activity of the B. subtilis 26D strain was shown, since the recombinant B. subtilis 26Dsfp line partially lost its ability to stimulate growth and completely lost its ability to suppress the reproduction of the greenbug aphid. Presowing treatment of wheat seeds with B. subtilis 26D strain led to a rapid accumulation of hydrogen peroxide, an increase in peroxidase activity, and a decrease in catalase activity in wheat leaves colonization by S. graminum. This probably caused the resistance of such plants to the pest. Using a recombinant B. subtilis 26Dsfp line, we proved that surfactin exactly influenced the redox status of plants colonized by S. graminum, changing the activity of enzymes and the content of hydrogen peroxide. These results prove that it is surfactin that makes significant changes in the redox status of plants, affecting the activity of enzymes and the content of hydrogen peroxide, and also plays a certain role in the induction of systemic resistance mediated by the B. subtilis 26D strain in wheat populated by the pest.
Keywords: Bacillus subtilis, surfactin, wheat, cereal aphid, systemic resistance.
DOI: 10.21515/1999-1703-106-323-328

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Authors:

1. Rumyantsev Sergey Dmitrievich, PhD in Biology, research associate, FSBSI "Institute of Biochemistry and Genetics of the Ufa Federal Research Center of the Russian Academy of Sciences".
2. Blagova Daria Konstantinovna, PhD in Biology, research associate, FSBSI "Institute of Biochemistry and Genetics of the Ufa Federal Research Center of the Russian Academy of Sciences".
3. Alekseev Valentin Yurievich, PhD student, FSBSI "Institute of Biochemistry and Genetics of the Ufa Federal Research Center of the Russian Academy of Sciences".
4. Veselova Svetlana Viktorovna, PhD in Biology, senior researcher, FSBSI "Institute of Biochemistry and Genetics of the Ufa Federal Research Center of the Russian Academy of Sciences".
5. Maksimov Igor’ Vladimirovich, DSc in Biology, professor, head of the Laboratory, Chief Researcher, FSBSI "Institute of Biochemistry and Genetics of the Ufa Federal Research Center of the Russian Academy of Sciences".