Труды Кубанского государственного аграрного университета


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2022, № 94

UDC: 632.937.15
GSNTI: 68.37.31

Strains of the genus Bacillus with fungistatic properties against Fusarium sp. in wheat agriculture

The potential of wheat agrocenoses on leached chernozem for the isolation of antagonist strains to the phytopathogen Fusarium sp. was studied. 6 out of 50 isolated bacterial isolates belonging to the genus Bacillus had fungistatic properties in relation to the test strain Fusarium oxysporum. The restriction analysis of the 16S part of the ribosomal gene (ARDRA method) was used to determine the affiliation of the selected strains to the biological species Bacillus subtillis. subsp. subtilis and Bacillus amyloliquefaciens.
Keywords: Bacillus, Fusarium, antagonistic activity, strain, RAPD, ARDRA method.
DOI: 10.21515/1999-1703-94-256-261

References:

  1. Алёшин, В. Н. Контроль заболеваний растений за счет индуцированной резистентности с помощью некоторых химических веществ и биоагентов / В. Н. Алёшин, Т. В. Першакова, Г. А. Купин // Плодоводство и виноградарство Юга России. - 2018. - № 53. - С. 113-143. - https://doi.org/10.30679/2219-5335-2018-5-53-113-143.
  2. Павлюшин, В. А. Фузариоз зерновых культур и опасность микотоксинов в России / В. А. Павлюшин // АгроСнабФорум. - 2017. - № 3. - С. 41-43.
  3. Brauer, V. S. Antifungal agents in agriculture: Friends and foes of public health / V. S. Brauer, C. P. Rezende, A. M. Pessoni, R. G. De Paula, K. S. Rangappa, S. C. Nayaka, F. Almeida // Biomolecules. - 2019. - Т. 9. - No. 10. - Р. 521.
  4. Cawoy, H. Lipopeptides as main ingredients for inhibition of fungal phytopathogens by Bacillus subtilis/amyloliquefaciens / H. Cawoy, D. Debois, L. Franzil, E. De Pauw, P. Thonart, M. Ongena // Microbial biotechnology. - 2015. - Т. 8. - No. 2. - Р. 281-295.- https://doi.org/10.1111/1751-7915.12238.
  5. Damalas, C. A. Eleftherohorinos I. G. Pesticide exposure, safety issues, and risk assessment indicators / C. A. Damalas, I. G. Eleftherohorinos // International journal of environmental research and public health. - 2011. - Т. 8. - No. 5. - Р. 1402-1419. - https://doi.org/10.3390/ijerph8051402.
  6. Falcäo, L. L. Antimicrobial and plant growth-promoting properties of the cacao endophyte Bacillus subtilis ALB 629 / L. L. Falcäo, J. O. Silva-Werneck, B. R. Vilarinho, J. P. Da Silva, A. W. V. Pomella, L. H. Marcellino // Journal of applied microbiology. - 2014. - Т. 116. - No. 6. - Р. 1584-1592. - https://doi.org/10.1111/jam.12485.
  7. Ghorbanpour, M. Mechanisms underlying the protective effects of beneficial fungi against plant diseases / M. Ghorbanpour, M. Omidvari, P. Abbaszadeh-Dahaji, R. Omidvar, K. Kariman // Biological Control. - 2018. - Т. 117. - Р. 147-157. - https://doi.org/10.1016/j.biocontrol.2017.11.006.
  8. Glick, B. R. Plant growth-promoting bacteria: mechanisms and applications / B. R. Glick // Scientifica. - 2012. - Т. 2012. - https://doi.org/10.6064/2012/963401.
  9. Janssen, E. M. Fusarium infection and trichothecenes in barley and its comparison with wheat / E.M. Janssen, C. Liu, H.J. Van der Fels-Klerx12 // World Mycotoxin Journal. - 2018. - Т. 11. - No. 1. - Р. 33-46. - https://doi.org/10.3920/WMJ2017.2255.
  10. Khan, N.Combating Fusarium infection using Bacillus-based antimicrobials / N. Khan, M. Maymon, A.M. Hirsch // Microorganisms. - 2017. - Т. 5. - No. 4. - Р. 75. - https://doi.org/10.3390/microorganisms5040075.
  11. Kopyltsov, S. Bacillus subtilis for biological protection of Taxus baccata L. in landscape gardens / S. Kopyltsov, A. Gneush // E3S Web of Conferences. - EDP Sciences, 2021. - Т. 285. - Р. 02002. - https://doi.org/10.1051/e3sconf/202128502002.
  12. Logan, N. A. Bacillus / N. A. Logan, P. D. Vos // Bergey's manual of systematics of archaea and bacteria. - 2015. - С. 1-163. - https://doi.org/10.1002/9781118960608.gbm00530.
  13. Lucas, J. A. The evolution of fungicide resistance /j. A. Lucas, N. J. Hawkins, B. A. Fraaije // Advances in applied microbiology. - 2015. - Т. 90. - Р. 29-92. - https://doi.org/10.1016/bs. aambs. 2014.09.001.
  14. Mahdhi, A. Probiotic properties of halophilic Bacillus strains enhance protection of Artemia culture against pathogenic Vibrio / A. Mahdhi, Z. Hmila, K. Chaieb, F. Kamoun, A. Bakhrouf // Aquatic biology. - 2011. - Т. 13. - No. 3. - Р. 225-231. https://doi.org/10.3354/ab00368.
  15. Nagy, A. Biological control of oyster mushroom green mould disease by antagonistic Bacillus species/ A. Nagy, L. Manczinger, D. Tombácz, L. Hatvani, J. Gyõrfi, Z. Antal // Biol. Control Fungal Bact. Plant Pathog. - 2012. - Т. 78. - Р. 289-293.
  16. Pavlushin, V. A. Fusarium of grain crops and the danger of mycotoxins in Russia / V. A. Pavlushin // AgroForum. - 2017. - No. 3. - Р. 41-43.
  17. Ntushelo, K. The mode of action of Bacillus species against Fusarium graminearum, tools for investigation, and future prospects / K. Ntushelo, L. K. Ledwaba, M. E. Rauwane, O. A. Adebo, P. B. Njobeh // Toxins. - 2019. - Т. 11. - No. 10. - Р. 606. - https://doi.org/10.3390/toxins 11100606.
  18. Shafi, J. Bacillus species as versatile weapons for plant pathogens: a review /j. Shafi, H. Tian, M. Ji // Biotechnology & Biotechnological Equipment. - 2017. - Т. 31. - No. 3. - Р. 446-459. - https://doi.org/10.1080/13102818.2017.1286950
  19. Shuping, D.S.S. The use of plants to protect plants and food against fungal pathogens: A review / D.S.S. Shuping, J.N. Eloff // African Journal of Traditional, Complementary and Alternative Medicines. - 2017. - Т. 14. - No. 4. - С. 120-127. -https://doi.org/10.21010/ajtcam.v14i4.14
  20. Zalila-Kolsi, I. Antagonist effects of Bacillus spp. strains against Fusarium graminearum for protection of durum wheat (Triticum turgidum L. subsp. durum) / I. Zalila-Kolsi, A.B. Mahmoud, H. Ali, S. Sellami, Z. Nasfi, S. Tounsi, K. Jamoussi // Microbiological Research. - 2016. - Т. 192. - Р. 148-158. - https://doi.org/10.1016/j.micres.2016.06.012
  21. Zhao, L. Screening and characterization of endophytic Bacillus and Paenibacillus strains from medicinal plant Lonicera japonica for use as potential plant growth promoters / L. Zhao, Y. Xu, X.H. Lai, C. Shan, Z. Deng, Y. Ji // Brazilian Journal of Microbiology. - 2015. - Т. 46. - Р. 977-989. https://doi.org/10.1590/S1517-838246420140024

Authors:

  1. Kopyltsov Sergey Vasilevich, PhD in Biology, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.
  2. Milovanov Aleksandr Valerievich, PhD in Biology, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.
  3. Yelisyutikova Anastasiya Vasilevna, student, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.
  4. Bazhenova Ekaterina Denisovna, student, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.