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2021, № 92

UDC: 577.214:631.8:633.11
GSNTI: 34.23.57

Changes in transcriptional activity of TaAgo2 and TaAgo4 genes in wheat plants at infection with Stagonospora nodorum Berk

RNA interference is a process of highly specific silencing of target RNA or DNA fragments using the RISC complex with a small RNA region complementary to this fragment at the transcription or translation stage, aimed, among other things, at protecting plants from biotic stress. Argonaute proteins (Ago) are one of the main catalytic components of this process, which forms plant resistance to biotic stress. In this work, we showed that infection with the fungus S. nodоrum did not significantly affect the level of transcripts of the TaAgo2 and TaAgo4 genes in wheat plants. Treatment with the bacterial strain Bacillus subtilis 26D increased the transcriptional activity of these genes and reduced the degree of fungal development by the priming mechanism.
Keywords: RNA interference, Triticum aestivum, Bacillus subtilis 26D, Stagonodpora nodorum Berk, gene transcriptional activity.
DOI: 10.21515/1999-1703-92-196-200

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Authors:

1. Shein Mikhail Yurievich, PhD student, Institute of Biochemistry and Genetics of Ufa Federal Research Center of Russian Academy of Sciences.
2. Burkhanova Guzel Fanilevna, PhD in Biology, Institute of Biochemistry and Genetics of Ufa Federal Research Center of Russian Academy of Sciences.
3. Merzlyakova Anastasia Yurievna, student, Institute of Biochemistry and Genetics of Ufa Federal Research Center of Russian Academy of Sciences.
4. Maksimov Igor Vladimirovich, DSc in Biology, Professor, Institute of Biochemistry and Genetics of Ufa Federal Research Center of Russian Academy of Sciences.