<<<  Back

2018, № 72

UDC: 635.21:631:52
GSNTI: 68.35.03

Restoration from viruses potato of varieties created in leningrad scientific research institute of agricultural science "Belogorka"

The aim of the research was to create a potato seed material that has cured of recovered from a viral infection in the clone selection nurseries. Pitomniki otbora for grades the Charodey and Lomonosovsky were formed in 2014 based on meristem material, by the sorte Nevsky in 2015. The selеctions are made frоm super-super elite. In the future, the sееd material was maintained in a healthy state with the use of clonal selection and a set of special and agrоtechnological tеchniques. Thеy are aimed at limiting the sprеаd of a viral infection. Its use makes it possible to maintain the clоnal mаteriаl in the nursеry оf the sеlеction prаctically frее from infection оf the X-virus of pоtаtо (XPV), S-virus (SPV). Y-virus (YPV) not is widely distributed in clones of varieties Lomonosovsky and Nevsky. Infection MPV, despite the same complex, is present in clonal material annually. Its manifestation is wavy in nature, when relatively favorable years are interspersed with outbreaks of viral infection. There is a sharp increase in the number of clones of infected MPV, which means that the material has to be healed again by intensives culling. In such years the complex of improving methods used does not ensure the maintenance of the material at the level achieved earlier. The increase in infectiousness of the MPV not in all years of observation can be associated with early and mass flight of aphids, the main vectors of this virus. An important role here is played by the latent infection of this virus. The use of ELISA for determining latent infection in summer is ineffective and clearly insufficient. At this time and this method reveals only a part of the viral clones. In another part of the clones, MPV is likely to be in a form unavailable for this method. Seed productivity of clones in different years was at the varieties Lomonosovsky 560-800, Charodey 520-700, Nevsky 700-1000 g / bush.
Keywords: M-potato virus, latent infection, enzyme immunoassay (ELISA), visual assessment
DOI: 10.21515/1999-1703-72-373-377

References:

1. Анисимов, Б. В. Фитопатогенные вирусы и их контроль в семеноводстве картофеля: практическое руководство. - Москва, 2004. - 80 с.
2. Инструкция по применению иммуноферментного диагностического набора для определения вирусов картофеля / ФГБНУ ВНИИКХ. - Коренево, 2016. - 8 с.
3. Писарев, Б. А. Семеноводство картофеля / Б. А. Писарев, Л. Н. Трофимец. - Москва: Россельхозиздат, 1982. - 240 с.
4. Шелабина, Т. А. Мониторинг динамики численности и видового состава тлей-переносчиков Y-вируса в условиях опытного поля Ленинградского НИИСХ «Белогорка» / Т. А. Шелабина, С. А. Завьялова // Аграрная Россия. - 2016. - № 9. - С. 2-5.
5. Динамика, численность, видовой состав тлей-переносчиков вирусов и качество семенного материала картофеля в первичных питомниках / Т. А. Шелабина, С. А. Завьялова, А. А. Кузнецов, А. И. Родионенков // Экспериментальные и теоретические исследования в современной науке: сб. ст. по матер. VI междунар. науч.-практ. конф., № 6 (6), Новосибирск: СибАК, 2017. - С. 40-50.

Authors:

1. Shelabina Tamara Alekseevna, Phd in Agriculture, leading researcher, Leningrad research institute for agricultural science «Belogorka».
2. Rodionenkov Alexander Ivanovich, research fellow, Leningrad research institute for agricultural science «Belogorka».
3. Kuznetsov Alexey Anatolyevich, junior researcher, Leningrad research institute for agricultural science «Belogorka».
4. Zavyalova Svetlana Alekseevna, senior researcher, Leningrad research institute for agricultural science «Belogorka».