Труды Кубанского государственного аграрного университета


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2022, № 97

UDC: 632.937.15:632.938.2+577.218
GSNTI: 68.37.13

The role of endophytic bacteria genus Bacillus in the regulation of transcription factors gene expression involved in the defense response of wheat against aphid Schizaphis graminum (Rond.)

One of the mechanisms contributing to the formation of plant resistance against pests is associated with triggeringby strains of Bacillus spp. induced systemic resistance (ISR), regulated by hormones - salicylic acid (SA), jasmonic acid (JA) and ethylene. A distinctive feature of ISR is the increased generation of reactive oxygen species (ROS), which activate transcription factors (TFs) that regulate hormonal signaling pathways. In our work, we studied the effect of bacteria B. subtilis 26D (Cohn.) (Bs 26D), B. thuringiensis B-5351 (Berliner) (Bt B-5351) and their mixture on the regulation of TF genes expression, regulators of SA-, JA, and ethylene signaling pathways, and the inductionsystemic resistance in wheat plants against the greenbug aphid Schizaphis graminum Rond. Our results showed that in the moderately susceptible wheat of cultivar SU colonized with the greenbug aphid, the SA signaling pathway was suppressed, and the JA/ethylene signaling pathways were activated by induction of expression of the TaMYC2, TaERF1, and TaEIN3 TF genes after 24 hours of feeding. Bacterial strains induced ISR through accumulation of ROS and induction of hormonal signaling pathways. The Bs 26D strain activated the SA signaling pathway through the induction of TaWRKY13 TF gene expression, and the Bt B-5351 strain induced JA/ethylene signaling pathways through the induction of TaWRKY53b and TaERF1 TF genes expression after 72 hours of feeding, which increased aphid mortality and plant endurance. An additive effect of strains composition was found, which manifested itself in the activation of the expression of all three TF genes. Thus, the important role of the TaWRKY13, TaWRKY53b, and TaERF1 TF genes in the induction resistance of wheat plants against the greenbug aphid has been shown.
Keywords: Bacillus spp, wheat, cereal aphid, systemic resistance, transcription factors.
DOI: 10.21515/1999-1703-97-124-130

References:

  1. Алексеев, В. Ю. Афицидная и иммуностимулирующая активность бактериальных липопептидов, продуцируемых штаммами Bacillussubtilis / В. Ю. Алексеев, С. Д. Румянцев, С. В. Веселова, Е. А. Черепанова, И. В. Максимов // Труды Кубанского государственного аграрного университета. - 2021. - № 93. - С. 168-173. DOI: 10.21515/1999-1703-93-168-173.
  2. Веселова, С. В. Бактерии рода Bacillus в регуляции устойчивости пшеницы к обыкновенной злаковой тле Schizaphis graminum Rond. / С. В. Веселова, Г. Ф. Бурханова., С. Д. Румянцев, Д. К. Благова, И. В. Максимов // Прикладная биохимия и микробиология. - 2019. - № 1. - Т. 55. - С. 56-63. DOI: 10.1134/ S0555109919010185.
  3. Веселова, С. В. Влияние хозяин-специфичного токсина SnTOX3 патогена Stagonosporanodorum на сигнальный путь этилена и редос-статус растений мягкой яровой пшеницы / С. В. Веселова, Г. Ф. Бурханова., Т. В. Нужная, С. Д. Румянцев, И. В. Максимов // Вавиловский журнал генетики и селекции. - 2019. - № 23(7). - С. 856-864. - DOI 10.18699/VJ19.559.
  4. Agarwal, P. WRKY: its structure, evolutionary relationship, DNA-binding selectivity, role in stress tolerance and development of plants / P. Agarwal, M. P. Reddy, J. Chikara // MolBiol Rep. - 2011. - Vol. 38. - P. 3883-3896. - DOI 10.1007/s11033-010-0504-5.
  5. Koch, K. G. Plant tolerance: a unique approach to control hemipteran pests / K. G. Koch, K. Chapman, J. Louis, T. Heng-Moss, G. Sarath // Front Plant Sci. - 2016. - Vol. 7. - Art. 1363. - DOI: 10.3389/fpls.2016.01363.
  6. Maksimov, I. V. Recombinant Bacillus subtilis 26DCryChS line with gene Btcry1Ia encoding Cry1Ia toxin from Bacillus thuringiensis promotes integrated wheat defense against pathogen Stagonospora nodorum Berk. and greenbug Schizaphis graminum Rond. / I. V. Maksimov, D. K. Blagova, S. V. Veselova, A. V. Sorokan, G. F. Burkhanova, E. A. Cherepanova, E. R. Sarvarova, S. D. Rumyantsev, V. Yu. Alekseev, R. M. Khayrullin // Biological Control. - 2020. - Vol. 144. - Art. 104242. - DOI: 10.1016/j.biocontrol.2020.104242.
  7. Miljaković, D. The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops / D. Miljakovic, J. Marinković, S. Balešević-Tubić // Microorganisms. - 2020. - Vol. 8(7). - Art. 1037. - DOI:10.3390/microorganisms8071037.
  8. Morkunas, I. Phytohormonal signaling in plant responses to aphid feeding / I. Morkunas, V. C. Mai, B. Gabrys // ActaPhysiol Plant. - 2011. - Vol. 33. - P. 2057-2073. - DOI 10.1007/s11738-011-0751-7.
  9. Pieterse, C. M. J. Hormonal modulation of plant immunity / C. M. J. Pieterse, D. van der Does, C. Zamioudis, A. Leon-Reyes, S. C. M. van Wees // Annu Rev Cell Dev Biol. - 2012. - Vol. 28. - P. 489-521. - DOI: 10.1146/annurev-cellbio-092910-154055.
  10. Rashid, M. H. Induction of systemic resistance against insect herbivores in plants by beneficial soil microbes / M. H. Rashid, Y. R. Chung // Front Plant Sci. - 2017. - Vol. 8. - Art. 1816. - DOI: 10.3389/fpls.2017.01816.
  11. Van Eck, L. The transcriptional network of WRKY53 in cereals links oxidative responses to biotic and abiotic stress inputs / L. Van Eck, R. M. Davidson, S. Wu, B.Y. Zhao, A.-M. Botha, J. E. Leach, N. L. V. Lapitan // FunctIntegr Genomics. - 2014. - Vol. 14. - P. 351-362. - DOI 10.1007/s10142-014-0374-3.
  12. Zheng, Z. Arabidopsis WRKY33 transcription factor is required for resistance to necrotrophic fungal pathogens / Z. Zheng, S. A. Qamar, Z. Chen, T. Mengiste // Plant J. - 2006. - Vol. 48(4). - P. 592-605. - DOI: 10.1111/j.1365-313X.2006.02901.x.

Authors:

  1. Rumyantsev Sergey Dmitrievich, PhD in Biology, research associate, FSBEI "Institute of Biochemistry and Genetics of the Ufa Federal Research Center of the Russian Academy of Sciences".
  2. Alekseev Valentin Yurievich, PhD student, FSBEI "Institute of Biochemistry and Genetics of the Ufa Federal Research Center of the Russian Academy of Sciences".
  3. Veselova Svetlana Viktorovna, PhD in Biology, senior Researcher, FSBEI "Institute of Biochemistry and Genetics of the Ufa Federal Research Center of the Russian Academy of Sciences".
  4. Burkhanova Guzel Fanilevna, PhD in Biology, research associate, FSBEI "Institute of Biochemistry and Genetics of the Ufa Federal Research Center of the Russian Academy of Sciences".
  5. Maksimov Igor’ Vladimirovich, DSc in Biology, professor, head of the Laboratory, Chief Researcher, FSBEI "Institute of Biochemistry and Genetics of the Ufa Federal Research Center of the Russian Academy of Sciences".