Труды Кубанского государственного аграрного университета

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2021, № 91

UDC: 579.26:579.22:579.64
GSNTI: 34.27.23, 34.31.37, 34.31.19

Plants and entomopathogenic bacteria Bacillus thuringiensis Interaction in the agrocenosis of Origanum vulgare L

Oregano can be promising for cultivation as an industrial scale, if it will be stable resistant to adverse environmental conditions and has a stability in the accumulation of valuable essential oil substances that determine the medicinal and nutritional value of this crop. This paper is devoted to the study of the entomopathogenic bacterium B. thuringiensis influence on metabolism and the main indicators of oregano plants physiological state. It was found that the most resistant to volatile antimicrobial fractions in O. vulgare samples No. 1, No. 2, and No. r-4 are spores of B. thuringiensis 888. The maximum activity of volatile fractions was noted for sample № r-4 in the phase of flowering (in 1,8 time to control). A significant increase in the antimicrobial activity of O. vulgare samples may be associated with an increase in the content of essential oil and the bactericidal secondary metabolites synthesized by them - carvacrol, γ-terpinene, and p-cymene. Treatment with the B. thuringiensis strain 888 increases the mass fraction of phenolic compounds in samples No. 2 and № r-4, respectively, by 24,9 and 29,8% compared to control and the formation of a persistent tendency towards the accumulation of reducing sugars in stems and leaves - up to 30,8% to control. The liquid spore culture of the B. thuringiensis 888 strain contributes to a 2-fold decrease in the chlorophyll content in sample № 2 and an increase in its content in the No. r-4 sample by a factor of 1,4.
Keywords: Origanum vulgare L, Bacillus thuringiensis, essential oil, reducing sugars, phenolic compounds, antioxidants, strain.
DOI: 10.21515/1999-1703-91-174-180


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  1. Kryzhko Anastasiia Vladimirovna, PhD in Agriculture, Federal State Budgetary Institution of Science “Research Institute of Agriculture of Crimea”.