2021, № 88
UDC: 632.7:575.116.4GSNTI: 68.37.29, 34.15.23
Design of the molecular markers to identify the higher taxonomic ranks of insects inhabiting agrocoenoses of the Krasnodar Territory (Insecta)
The design of molecular primer pairs for 4 orders and 24 families of the common insects widely distributed in the agrocoenoses of Krasnodar Territory is described on the basis of 8S rRNA and 28S rRNA.Keywords: DNA, PCR, primers, higher insects taxa, agricultural pests, predatory, entomophages, gut content, Krasnodar Territory.
DOI: 10.21515/1999-1703-88-111-119
References:
- Китаев, К. А. Молекулярно-генетические методы анализа хищничества среди насекомых в агроценозах / К. А. Китаев, М. Б. Удалов, Г. В. Беньковская // Экологическая генетика. - 2011 - T. 9 (4). - C. 15-24.
- Хомицкий, Е. Е. Создание и тестирование in silico молекулярных маркеров с целью идентификации 16 видов насекомых-вредителей сельского хозяйства в Краснодарском крае / Е. Е. Хомицкий, А. С. Замотайлов, Д. С. Савенкова, А. В. Милованов, А. И. Белый // Труды Кубанского государственного аграрного университета. - 2019. - Вып. 5 (80). - С. 200-208. - https://doi.org/:10.21515/1999-1703-80-200-208.
- Campbell, B. C. Evolutionary origin of whiteflies (Hemiptera: Sternorrhyncha: Aleyrodidae) inferred from 18S rDNA sequences / B. C. Campbell, J. D. Steffen, B. C. Campbell, R. J. Gill // Insect molecular biology. - 1994. - Vol. 3. - No 2. - P. 73-88. - https://doi.org/10.1111/j.1365-2583.1994. tb00154.x.
- Frati, F. Evolution of the mitochondrial cytochrome oxidase II gene in Collembola / F. Frati, C. Simon, J. Sullivan, D.L. Swofford // Journal of Molecular Evolution. - 1997. - Vol. 44. - No 2. - P. 145-158. - https://doi.org/10.1007/ PL00006131.
- Geer, L. Y. The NCBI biosystems database / L. Y. Geer, A. Marchler-Bauer, R. C. Geer, L. Han, J. He, S. He, C. Liu, W. Shi, S.H. Bryant // Nucleic acids research. - 2010. - Vol. 38. - Suppl. 1. - P. D492-D496. - https://doi.org/10.1093/nar/ gkp858.
- Golub, N. V. New data on karyotypes of lace bugs (Tingidae, Cimicomorpha, Hemiptera) with analysis of the 18S rDNA clusters distribution / N. V. Golub, V. B. Golub, V. G. Kuznetsova // Comparative Cytogenetics. - 2018. - Vol. 12. - No 4. - P. 515. - https://doi.org/10.3897/Comp Cytogen.v12i4.30431.
- Kibbe, W. A. OligoCalc: an online oligonucleotide properties calculator // Nucleic acids research. - 2007. - Vol. 35. - Suppl. 2. - P. W43 - W46. - https://doi.org/10.1093/nar/gkm234.
- Mioduchowska, M. Instances of erroneous DNA barcoding of metazoan invertebrates: Are universal cox1 gene primers too "universal"? / M. Mioduchowska, M. J. Czyż, B. Gołdyn, J. Kur, J. Sell // PLoS One. - 2018. - Vol. 13. - No 6. - P. e0199609. - https://doi.org/10.1371/journal.pone. 0199609.
- Untergasser, A. Primer3 - new capabilities and interfaces / A. Untergasser, I. Cutcutache, T. Koressaar, J. Ye, B. C. Faircloth, M. Remm, S. G. Rozen // Nucleic acids research. - 2012. - Vol. 40. - No 15. - P. e115. - https://doi.org/10.1093/ nar/gks596.
Authors:
- Khomitskyi Evgeniy Evgenievich, post-graduate student, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.
- Zamotajlov Alexandr Sergeevich, Dr. Sc., Professor, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.
- Savenkova Dar'ya Sergeevna, student, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.
- Milovanov Aleksandr Valerievich, PhD in Biology, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.
- Belyi Alexandr Ivanovich, PhD in Agriculture, Assistant Professor, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.