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    2019, № 80

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    The effect of protein level and its breakdown on the use of nitrogen feed, the supply of essential amino acids and milk production of holstein fresh cows

    The materials presented in the article are a continuation of the experiment of the previously described material in “Proceedings of KubSAU” (Ryadchikov V.G. et al. 2019) The purpose of this study was to study the effect of different levels of protein and its degradability on the efficiency of using feed N, the formation of microbial protein, the release of amino acids into the duodenum, the provision of diets with metabolic essential amino acids and milk production of cows. In the experiment, there were 4 Holstein first-calf cows during lactation 45 ± 10 days from calving, surgically equipped with rumen and duodenal cannulas. Four diets were evaluated for effectiveness. The 1st and 2nd diets contained 15.7% CP. The ratio of RUP:RDP = 7,6: 8,1% DM (50:50% CP), in 2 diet, the ratio of RUP: RDP = 5,4: 10,3% DM, (35:65% CP); in the 3rd and 4th diets, the CP content is 17.7%, in the 3rd ration the ratio of RUP:RDP is 9.1: 8.7% DM (50:50% CP), in the 4th ration the ratio of RUP:RDP is 6.3: 11.4% DM (35:65% CP). Feeding animals was carried out according to the Latin square 4 ½ 4. The experiment consisted of 4 periods of 22 days each, with 12 days being the period of adaptation to the new diet, 10 days - milk yield accounting period, sampling of digest, feces, urine, blood. There was a trend of higher apparent and true digestibility of DM and OM in the rumen, the same tendency to increase digestibility by 2% in the whole digestive tract one the diets with the ratio RUP:RDP = 35:65. The most effective use of feed, RUP nitrogen, and RDP nitrogen was observed in cows on a diet with 15,7% DM, with the ratio of RUP:RDP = 35:65% DM. On a diet with 15,7% DM and a ratio of RUP:RDP = 50:50% CP, a decrease in DM (p <0.05). intake and milk production was observed on 4 diet. The maximum milk production (p <0.05) was obtained on a 3 ration of 39.4 kg / d with a ratio of RUP:RDP = 50: 50% CР. The provision of diets with essential amino acids for lysine amounted to 95.3% in diets 2 and 3, methionine deficiency was about 15%. A less satisfactory balance of diets for metabolic essential amino acids was observed on diets 1 and 4. There is a direct correlation between the level of RRB in diets and the growth of microbial biomass (p <0.05). With the optimal ratio of protein fractions RUP:RDP and the optimal balance of metabolic essential amino acids there is a real opportunity to reduce the protein level in the diet of cows to 15.7%.
    Keywords: Lactating cows, protein level, protein breakdown, essential amino acids, nitrogen use, milk productivity
    DOI: 10.21515/1999-1703-80-260-279

    References:

    1. Определение микробного азота в дуоденальном содержимом по пуриновым основаниям / C. 143-145. В к-н «Экспериментально-прикладная физиология пищеварения жвачных животных», Боровск, 2019, 448 с.
    2. Рядчиков, В. Г. Влияние уровня и распадаемости белка в рубце на обмен азота и молочную продуктивность голштинских коров в ранней лактации / В. Г. Рядчиков, О. Г. Шляхова, А. А. Тантави, Н. С. Филева // Труды Кубанского государственного аграрного университета. - Краснодар. - 2018. - № 75. - С. 154-166.
    3. Рядчиков, В. Г. Потребность лактирующих коров в незаменимых аминокислотах / В. Г. Рядчиков, О. Г. Шляхова, А. Тантави, Н. С. Филева // Политематический сетевой электронный научный журнал Кубанского государственного аграрного университета. - Краснодар. - 2019. - № 148. - С. 162-200.
    4. Рядчиков, В. Г. Аминокислотное питание молочных коров / В. Г. Рядчиков. - Матер. конф., посвящ. 120-летию М.Ф. Томмэ «Фундаментальные и прикладные аспекты кормления сельскохозяйственных животных и технология кормов». - 14-16 июня 2016 г. - С. 400-404. - Дубровицы. 2016.
    5. Рядчиков, В. Г. Качество белка для жвачных / В. Г. Рядчиков. - С. 262-272 В кн.: Основы питания и кормления сельскохозяйственных животных. Краснодар: КГАУ, 2013. - 616 с.
    6. Рядчиков, В. Г. Нормы и рационы для молочного скота / / В. Г. Рядчиков. - В кн. «Основы питания и кормления сельскохозяйственных животных». - Изд. «Лань». Санкт-Петербург. - 2015. - С. 277-290.
    7. Рядчиков, В. Г. Потребность лактирующих коров в незаменимых аминокислотах / В. Г. Рядчиков, О. Г. Шляхова, А. Тантави, Н. С. Филева // Политематический сетевой электронный научный журнал Кубанского государственного аграрного университета. - Краснодар. - 2019. - № 150. - С. 195-235.
    8. Рядчиков, В. Г. Концентрация аминокислот в плазме крови у коров в переходный период, трансформация обменного белка, лизина и метионина в их компоненты молока в зависимости от уровня белка в рационе / В. Г. Рядчиков, О. Г. Шляхова // Тр. / КубГАУ. - 2013. - № 5 (44). - С. 212-225.
    9. Харитонов, Е. Л. Повышение протеиновой питательности кормов для молочных коров: метод. положения / Е. Л. Харитонов, Д. Г. Погосян. - Боровск:ВНИИФБиП с.-х. животных, 2011. - 64 с.
    10. Харитонов, Е. Л. Принципы расчета образования субстратов и метаболитов в желудочно-кишечном тракте жвачных животных / Е. Л. Харитонов, А. М. Материкин // Доклады Российской академии сельскохозяйственных наук. - 2001. - № 3. - С. 33-37.
    11. Харитонов, Е. Л. Физиология и биохимия питания молочных коров / Е. Л. Харитонов. - Гл. 4 «Протеиновое питание молочных коров». С. 75-139. - Монография. - Боровск. - Изд-во «Оптима Пресс». - 2011.
    12. Bahrami-yekdangi, H. Effects of decreasing metabolizable protein and rumen-undegradable protein on milk production and composition and blood metabolites of Holstein dairy cows in early lactation / H. Bahrami-yekdangi, M. Khorvash, G. R. Ghorbani // J. Dairy Sci. - 2014. - Vol. 97. - P. 3707-3714. Doi: 10.3168/jds.2013-6725.
    13. Bahrami-yekdangi, M. Reducing crude protein and rumen degradable protein with a constant concentration of rumen undegradable protein in the diet of dairy cows: Production performance, nutrient digestibility, nitrogen efficiency, and blood metabolites1 / M. Bahrami-yekdangi, G. R. Ghorbani, M. Khorvash, M. A. Khan & M. H. Ghaffari // Journal of Animal Science, 94(2), 718-725. Doi: 10.2527/jas.2015-9947.
    14. Batista, E. D. Effects of varying ruminally undegradable protein supplementation on forage digestion, nitrogen metabolism, and urea kinetics in Nellore cattle fed low-quality tropical forage / E. D. Batista, E. Detmann, E. C. Titgemeyer, S. C. Valadares Filho (et al.) // J. Anim. Sci. 2016. - Vol. 94(E. Suppl.). - P. 94-201. Doi: 10.2527/jas.2015-9493.
    15. Broderick, G. A. Effect of dietary protein concentration and degradability on response to rumen-protected methionine in lactating cows / G. A. Broderick, M. J. Stevenson, R. A. Patton // J. Dairy Sci. - 2009. - Vol. 92. - P. 2719-2726. Doi: 10.3168/jds.2008-1277.
    16. Clark, J. H. Symposium: Nitrogen metabolism and amino acid nutrition in dairy cattle. Microbial Protein Synthesis and flows of nitrogen fractions to the duodenum of dairy cows // J. H. Clark, Klusmeyer and M. R. Cameron // Journal of Dairy Science. - 1992. 75:2304 - 2323.
    17. CNCPS. 2000. The Cоrnell University Nutrient Management Planning System. The net carbоhydrate and prоtein system fоr evaluating herd nutritiоn and nutrient eхcretiоn. CNCPS versiоn 4.0, Nоvember 3rd, 2000. Mоdel Dоcumentatiоn. DOI:10.1016/j.anifeedsci.2003.10.006.
    18. Duncan, D. B. T tests and intervals for comparisons suggested by the data. Biometrics 31:339-59, 1975. http://garfield. library.upenn.edu /classics1977 /A1977 DM02600001.pdf.
    19. Dоepel, L. Milk Protein Synthesis as a Function of Amino Acid Supply / L. Dоepel, D. Pacheco and M. Hanigan // Journal of Dairy Science. 2004.- 87(5):1279-97. DOI: 10.3168/jds.S0022-0302(04)73278-6
    20. Dоepel, L. Changes in prоductiоn and mammary metabоlism оf dairy cоws in respоnse tо essential and nоnessential aminо acid infusiоns / L. Dоepel and H. Lapierre // J. Dairy Sci. 2010.- Vоl. 93. P. 3264-3274. https:// Dоi.оrg/ 10.3168/ jds.2009 - 3033. Dоi.оrg/ 10.3168/ jds. 2009. - 3033.
    21. Paula, E. M. Effects оf replacing sоybean meal with canоla meal оr treated canоla meal оn ruminal digestiоn, оmasal nutrient flоw, and perfоrmance in lactating dairy cоws / E. M. Paula, G. A. Brоderick, M. A. C. Danes(et al.) // J. Dairy Sci. - 2018. - Vоl. 101. - P. 1-12. Doi: 10.3168/jds.2017-13392. Epub 2017 Nov 10.
    22. Fessenden, S. W. Rumen digestion kinetics, microbial yield, and omasal flows of nonmicrobial, bacterial, and protozoal amino acids in lactating dairy cattle fed fermentation by-products or urea as a soluble nitrogen source / S. W. Fessenden, T. J. Hackmann, D. A. Ross, E. Block, A. Foskolos, M.E. Van Amburgh // Journal of Dairy Science.102(4):3036-3052. Doi:10.3168/jds.2018-15448.
    23. Huhtanen, P. А. A meta-analysis of the effects of dietary protein concentration and degradability on milk protein yield and milk n efficiency in dairy cows / P. A. Huhtanen and A. N. Hristov // Journal of Dairy Science. - 2009. 92:3222-3232. Doi: 10.3168/jds.2008-1352.
    24. Ipharraguerre, I. R. Rumen fermentation and intestinal supply of nutrients in dairy cows fed rumen-protected soy products / I. R. Ipharraguerre, J. H. Clark, D. E. Freeman // Journal of Dairy Science. - 2005. 88:2879-2892. Doi 10.3168/jds.S0022-0302(05)72969-6.
    25. Kaufman, J. D. Lowering rumen-degradable protein and rumen-undegradable protein improved amino acid metabolism and energy utilization in lactating dairy cows exposed to heat stress / J. D. Kaufman, K. G. Pohler, J. T. Mullinks and A. G. Rius // Journal of Dairy Science. - 2017. 101:386 - 395. Doi: 10.3168/jds.2017-13341.
    26. Kaufman, J. D. Lowering rumen-degradable protein maintained energy-corrected milk yield and improved nitrogen-use efficiency in multiparous lactating dairy cows exposed to heat stress / J. D. Kaufman, K. R. Kassube & A. G. Ríus // Journal of Dairy Science. - 2018. 100(10), 8132-8145.
    27. Lapierre, H. Estimation of histidine requirement in lactating dairy cows // H. Lapierre, D. R. Ouellet and G. E. J. Lobley // Anim. Sci Vol. - 2014. - N. 92, Vol. 97. - P. 172-188. Doi:10.3168/jds.S00220302(06)72359-1.
    28. Lapierre, H. What is the True Supply of Amino Acid for a Dairy Cow? // H. Lapierre, D. Pacheco, R. Bertiaume, D. R. Ouellet, C. G. Schwab, P. Dubreuil, G. Holtrop and G. E. Lobley // Journal of Dairy Science. - 2006. 89 (E. Suppl.): Е1-Е14.
    29. Martineau, R. Feeding canola meal to dairy cows: A meta-analysis on lactational responses / R. Martineau, D. R. Ouellet, H. Lapierre // J. Dairy Sci. - 2013. - Vol. 96. - P. 1701-1714. DOI: 10.3168/jds.2012-5740.
    30. Martins, C. M. M. R. Effect of dietary crude protein degradability and corn processing on lactation performance, milk protein composition, and stability / C. M. M. R. Martins, D. C. M. Fonseca, B. G. Alves, M. A. Arcari, G. C. Ferreira, K. C. Welter, C. A. F. Oliverira, F. P. Renno and M. V. Santos // Journal of Dairy Science. - 2019. 102:1 - 14.
    31. Moore, S. 1963. On the determination of cysteine as cysteic acid / S. Moore // J. Biol. Chem. 238:235-237.
    32. Mutsvangwa, T. Effects of dietary crude protein and rumen-degradable protein concentrations on urea recycling, nitrogen balance, omasal nutrient flow, and milk production in dairy cows. / T. Mutsvangwa, K. L. Davies, J. J. McKinnon // Journal of Dairy Science. - 2016. 99(8), 6298-6310. DOI:10.3168/jds.2016-10917.
    33. NRC - Natiоnal Research Cоuncil. Nutrient requirements оf dairy cattle. 7th ed. Nutrient requirements оf dоmestic animals. - Washingtоn: Natiоnal Academy Press, 2001. - 547 p.
    34. Ouellet, D. R. Effect of method of conservation of timothy on endogenous nitrogen flows in lactating dairy cows / D. R. Ouellet, R. Berthiaume, G. Holtrop // J. Dairy Sci. - 2010. - Sep. 93(9). - P. 4252-4261.
    35. Ouellet, D. R. Effect of dietary fiber on endogenous nitrogen flows in lactating dairy cows / D. R. Ouellet, M. Demers, G. Zuur // J. Dairy Sci. - 2002. - Vol. 85. - P. 3013-3025.
    36. Ouellet, D. R. CNCPS. Contribution of endogenous secretions and urea recycling to nitrogen metabolism / D. R. Ouellet, D. Valkeners, G. Holtrop // Proc. Cornell Nutrition Conference for feed manufacturers Dpt. Anim. Science, Cornell University. - NY, 2007. - P. 1.
    37. Ouellet, D. R. Endogenous nitrogen secretions in dairy cows: transaction along the digestive tract and implication in amino acid requirements Agriculture and Agri-Food Canada / D. R. Ouellet and H. Lapierre // 29th Discover conference "Amino acid requirements of dairy cattle". - 2014. may 26-29. - Р. 10.
    38. Reynal, S. M. Effect of Feeding Protein Supplements of Differing Degradability on Omasal Flow of Microbial and Underrated Protein // S. M. Reynal and G. A. Broderick // Journal of Dairy Science. - 2005. - 88:4045-4046.
    39. Reynal, S. M. Effect of Feeding Protein Supplements of Differing Degradability on Omasal Flow of Microbial and Undegraded addedein / S. M. Reynal, G. A. Broderuck, S. Ahvenjarvi, and P. Huhtanen // J. Dairy Sci. - 2003. Vol. - 86:1292-1305. DOI: 10.3168/jds.S0022-0302(03) 73666-2.
    40. SAS Institute. SAS/STAT user's guide // Statistics, version 6, 4th ed. - 2003. - Vol. 2. https:// support.sas.com / documentation / onlinedoc /stat / 143/ stathpug.pdf.
    41. Savari, M. Effects of rumen-degradable protein:rumenundegradable protein ratio and corn processing on production performance, nitrogen efficiency, and feeding behavior of Holstein dairy cows / M. Savari, M. Khorvash, H. Amanlou [et al.] // J. Dairy Sci. - 2018. - Vol. 101(2). - P. 1111-1122. DOI:10.3168/jds.2017-12776.
    42. Schwab, C. G. Maximizing milk components and metabolizable protein utilization through amino acid formulation / C. G. Schwab and G. N. Foster // Proceeding of the Cornel l Nutrition Conference for Need Manufacturers. New York. - October 20-22, 2009. https:// formulate2corecomponents.com/files/Cornell_Nutrition_Conference_2009.pdf.
    43. Sok, M. Amino acid composition of rumen bacteria and protozoa in cattle / M. Sok, D. R. Ouellet, J. L. Firkins // J. Dairy Sci. - 2017. - Vol. 100. - P. 5241-5249. DOI: 10.3168/jds.2017-12647.
    44. Tacoma, R. Ratio of dietary rumen degradable protein to rumen undegradable protein affects nitrogen partitioning but does not affect the bovine milk proteome produced by mid-lactation Holstein dairy cows / R. Tacoma, J. Fields, D. B. Ebenstein, Y.-W. Lam & S. L. Greenwood (2017) // Journal of Dairy Science, 100(9), 7246-7261.
    45. Van Sоest, P. J. 1982. Nutritiоnal ecоlоgy оf the mminnnt / P. J. Van Sоest. - Cоmstоck, CоmeU Univ. Press, Itbaca, NY. https:// trove.nla.gov.au/version/13770830.
    46. Wang, C. Effects of dietary supplementation of methionine and lysine on milk production and nitrogen utilization in dairy cows / C. Wang, H. Y. Liu, Y. M. Wang [et al.] // J. Dairy Sci. - 2010. - Vol. 93. - P. 3661-3670. DOI: 10.3168/jds.2009-2750.
    47. White, R. R., 1. Evaluation of the National Research Council dairy model and derivation of new prediction equations. 2. Rumen degradable and undegradable protein / R. R. Y. White, J. L. Roman-Garcia, P. Firkins, M. J. Kononoff, H. VandeHaar, T. McGill Tran, R. Garnett, and M. D. Hanigan // Journal of Dairy Science. - 2017. 100:3611-3627. DOI: 10.3168/jds.2015-10801.

    Authors:

    1. Ryadchikov Victor Georgievich, DSc in Biology, рrofessor, Academician of RAN, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.
    2. Soldatov Anatoly Alekseevich, DSc in Agriculture, рrofessor, director, ООО Premix.
    3. Kharitonov Evgeny Leonidovich, DSc in Biology, рrofessor, director, All-Russian Research Institute of Physiology, Biochemistry and Animal Nutrition - a branch of the Federal State Budget Scientific Institution "Federal Scientific Center for Livestock - VIZH named after academician L.K. Ernst".
    4. Tantawi Abuelkassem, graduate student, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.
    5. Fileva Nina Sergeevna, graduate student, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.
    6. Shlyakhova Oksana Germanovna, Phd in Biology, associate Professor, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.
    7. Dmitrienko Stanislav Nikolaevich, Phd in Biology, associate Professor, Leading Specialist, Federal State Budgetary Educational Institution of Higher Education “I.T. Trubilin Kuban State Agrarian University”.

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