Труды Кубанского государственного аграрного университета


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2019, № 78

UDC: 632.937.12+595.792.17
GSNTI: 34.33.19, 34.35.17, 68.37.13

Molecular genetic analysis of the structure variability of entomophages populations on the example of the parasitic hymenoptera Habrobracon hebetor Say and the predatory bug Perillus bioculatus F

To study the trophic relationships of entomophages and the genetic conditionality of this trait a comparative molecular-genetic analysis of the structure of the parasitic hymenoptera Habrobracon hebetor Say and the predatory bug Perillus bioculatus F. populations differing in their geographic location, food preferences has been carried out using the polymerase chain reaction (PCR) method for DNA markers. Taking for basis the results of the RAPD- (random amplification of polymorphic DNA) and ISSR (inter simple sequence repeat) PCR-analysis, we assessed the variability of the structure of P. bioculatus geographical populations from different areas of the Krasnodar Territory. In Krasnoarmeisky district and the city of Krasnodar, the genetic identity of perillus populations was 0.99; when compared with the population of Krymskiy district, this value was significantly lower - 0.67, which indicates the presence of geographical variation in the genetic structure and genetic diversity of perillus populations in the Krasnodar Territory. The conducted studies confirm the plasticity of the species, which gives grounds to continue its acclimatization on the territories of the modern habitat of the Colorado beetle. A molecular-genetic analysis of the geographical populations of the Habrobracon hebetor entomophage (from Krasnodar, Russia and Chimkent, Kazakhstan) was carried out. DNA markers specific for the Krasnodar and Chimkent populations of the bioagent were identified, allowing to distinguish one population from another, that is, to identify the studied Habrobracon populations. For the Krasnodar population, these are RAPD markers: 550 bp. (UBC519), 500 and 700 bp (OPA05); 1100, 1200 and 1300 bp (OPA10); 220 and 800 bp (OPB04); 880 bp (OPB01); for the Chimkent population: 400, 600 and 1200 bp (UBC519); 600 and 950 bp (OPA10); 800 bp (OPB01). The differences in the trophic relationships of the populations of the studied entomophages were found out to be genetically determined.
Keywords: Ectoparasite Habrobracon hebetor Say, predatory bug Perillus bioculatus F, PCR analysis, population, genetic similarity, entomophages
DOI: 10.21515/1999-1703-78-75-79

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Authors:

  1. Besedina Ekaterina Nikolaevna, PhD in Biology, Senior Researcher, FSBSI «All-Russian Scientific Research Institute of Biological Plant Protection».
  2. Agasieva Irina Sergeevna, PhD in Biology, Leading researcher, FSBSI «All-Russian Scientific Research Institute of Biological Plant Protection».
  3. Ismailov Vladimir Yakovlevich, PhD in Biology, Deputy Director on Science and Innovations, FSBSI «All-Russian Scientific Research Institute of Biological Plant Protection».
  4. Kil Vladimir Ilich, DSc in Biology, Professor of the Geo-ecology and Environmental Management Department, Federal State Budgetary Educational Institution of Higher Education «Kuban State University».