Труды Кубанского государственного аграрного университета

<<<  Back

2018, № 72

UDC: 575+633.11:632.488
GSNTI: 68.37.07

Diagnosis Tsn1 gene of Pyrenophora tritici-repentis in wheat varieties and their resistance to races of the pathogen

Recently, Тan spot, caused by Pyrenophora tritici-repentis (Died.) Drechsler, has occupied larger area on the territory of Russia. Due to the wide spread and economic importance of this disease, the search for varieties resistant to the pathogen is of high priority. This study presents the results of the diagnosis of the functional allele of the Tsn1 gene of P. tritici-repentis, responsible for the development of necrosis (Ptr ToxA), in 27 varieties of winter wheat of Russian selection. Using the PCR method with primers for the Xfcp394 marker, the Tsn1 gene allele was found in 5 samples (18.5% of the studied amount): Agat Donskoy, Zimtra, Beauty, Marathon, Pervitsa. Under greenhouse conditions in the germination phase, the wheat varieties were evaluated for resistance to the widespread races of the pathogen in the territory of Russia. Samples of the resistant wheat were determined: 13 samples (or 48.1% of the studied amount) to the 1st race (forming two toxins: Ptr Tox A, Ptr Tox C), 27 samples (100,0%) to the 2nd race (forming the toxin Ptr Tox A), 2 samples (7.4%) to the 8th race (forming three toxins: Ptr Tox A, Ptr Tox C, Ptr Tox B). This study showed that the studied varieties possess a race-specific resistance. Analysis of the data obtained indicates that the varieties identified with PCR method as ToxA-resistant were susceptible to different races of the fungal isolate producing other toxins (ToxB and ToxC). The obtained results indicate the need for an integrated approach to the study of the resistance of wheat samples to P. tritici-repentis.
Keywords: Pyrenophora tritici-repentis, Tsn1 gene, race, wheat, stability
DOI: 10.21515/1999-1703-72-206-210


  1. Желтая пятнистость листьев пшеницы (возбудитель Pyrenophora tritici-repentis (Died.) Drechsler) / Г. В. Волкова, О. Ю. Кремнева, А. Е. Андронова, В. Д. Надыкта. - М.: АМА-ПРЕСС, 2012, - С. 62-82.
  2. Кремнева, О. Ю. Желтая пятнистость листьев пшеницы и влияние предшественника на ее развитие / О. Ю. Кремнева, Г. В. Волкова, И. Б. Попов // Труды Кубанского государственного аграрного университета. - 2014. - № 47. - С. 79-84.
  3. Кремнева, О. Ю. Структура популяции возбудителя желтой пятнистости листьев пшеницы на Северном Кавказе по вирулентности и элементы биологизированной защиты от патогена: дис..… канд. биол. наук / Кремнева О. Ю.; Кубанский государственный аграрный университет. - Краснодар, 2007. - С. 78-84.
  4. Селективное влияние сортов пшеницы с геном Tsn1 на формирование популяции возбудителя желтой пятнистости Pyrenophora tritici-repentis / Н. В. Мироненко, О. А. Баранова, Н. М. Коваленко, О. С. Афанасенко, Л. А. Михайлова // Вестник защиты растений. - 2017. - Вып. 3 (93). - С. 23-27.
  5. Михайлова, Л. А. Популяции Pyrenophora tritici-repentis на Северном Кавказе и северо-западе России: расовый состав и динамика вирулентности / Л. А. Михайлова, Н. В. Мироненко, Н. М. Коваленко // Микология и фитопатология. - 2014. - Т. 48, № 6. - С. 393-400.
  6. A unique wheat disease resistance-like gene governs effector-triggered susceptibility to necrotrophic pathogens / J. D. Faris, Z. Zhang, H. J. Lu, S. W. Lu, L. Reddy // Proc. Natl. Acad. Sci. USA. - 2010. - Vol. 107. - P. 13544-13549.
  7. Discoloration of wheat kernels by Pyrenophora tritici-repentis / M. R. Fernandez, R. M. De Pauw, J. M. Clarke, S. L. Fox // Canadian Journal of Plant Pathology. - 1998. - Vol. 20. - P. 380-383.
  8. The identification of two new races of Pyrenophora tritici-repentis from the host centre of diversity confirms a one to one relationship in tan spot of wheat / L. Lamari, S. E. Strelkov, A. Yahyaoui, J. Orabi, R. B. Smith // Phytopathology. - 2003. - Vol. 93. - Р. 391-396.
  9. Murray, H. G. Rapid isolation of high molecular weight DNA / H. G. Murray, W. F. Thompson // Nucl. Acids Res. - 1980. - Vol. 8. - P. 321-4325.
  10. Differential effector gene expression underpins epistasis in a plant fungal disease / H. T. T. Phan, K. Rybak, E. Furuki [et al.] // The Plant Journal. - 2016. - Vol. 87. - P. 343-354.
  11. Shabeer, A. Tan spot effects on yield and yield components relative to growth stage in winter wheat / A. Shabeer, W. W. Bockus // Plant Disease. - 1988. - Vol. 72. - P. 599-602.
  12. Development, identification, and validation of markers for marker-assisted selection against the Stagonospora nodorum toxin sensitivity genes Tsn1 and Snn2 in wheat / Z. Zhang, T. L. Friesen, K. J. Simons, S. S Xu, J. D. Faris // Mol. Breed. - 2009. - Vol. 23. - P. 35-49.


  1. Kremneva Oksana Yur'yevna, PhD in Biology, leading researcher of the laboratory; Тhe laboratory of the immunity of cereal crops to fungal diseases, Federal State Budget Scientific Institution "All- Russian research Institute biology plant protection".
  2. Volkova Galina Vladimirovna, DSc in Biology, head of the laboratory; Тhe laboratory of the immunity of cereal crops to fungal diseases, Federal State Budget Scientific Institution "All- Russian research Institute biology plant protection".