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2018, № 72

UDC: 57.084.1:635.153
GSNTI: 62.33.29

Introduction into in vitro culture and callusogenesis induction by winter round black raddish (Raphanus sativus L. var. niger)

Healing properties of black radish are highly appreciated: it is rich in enzymes, carotene, vitamin C, choline, magnesium, essential oils, proteins, fats. Glucosinolates and their decomposition products (with antioxidant capacity) can prevent the gallstones formation in the gallbladder and reduce the bile cholesterol secretion. Radish can have antidiabetic activity, influencing the hormones that affect glucose haemostasis. A promising way to preserve valuable genotypes and obtain secondary metabolites that have a high biological activity is the cell and tissue culture. In this work it was found that 5-minute sterilization with a 5% solution of sodium hypochlorite ensures a high yield of aseptic plants. In studying the induction of callusogenesis (as a method for obtaining a wide range of genotypes), the highest efficiency of this process is shown on the Murashige and Skoog nutrient medium with the addition of 2 mg/l 2,4-dichlorophenoxyacetic acid and 0,5 mg/l benzyladenine.
Keywords: Black radish, in vitro culture, seed sterilization, germination, callusogenesis
DOI: 10.21515/1999-1703-72-165-167

References:

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Authors:

1. Kalugina Yuliya Evgenevna, BSc-student, Genetics, Biotechnology, Federal State Budgetary Educational Institute of Higher Education “Russian State Agrarian University - Moscow Timiryazev Agricultural Academy”.
2. Malova Anastasiya Ruslanovna, BSc-student, Federal State Budgetary Educational Institute of Higher Education “Russian State Agrarian University - Moscow Timiryazev Agricultural Academy”.
3. Cherednichenko Mikhail Yuryevich, PhD in Biotechnology, Federal State Budgetary Educational Institute of Higher Education “Russian State Agrarian University - Moscow Timiryazev Agricultural Academy”.