Труды Кубанского государственного аграрного университета

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2017, № 67

UDC: 575.1: 632.4: 634.8.09
GSNTI: 34.23.57

Detection of the resistance gene Rpv3 in grapevine cultivars using DNA- markers

One of the most common and harmful diseases of the vine is mildew. The cultivation of resistant genotypes makes it possible to effective control the spread of the disease. European V. vinifera grapevines cultivars are unstable to mildew, donors of resistance mainly - North American species and V. amurensis. The breeding of resistant cultivars is based on interspecific hybridization. To date, more than fifteen major and minor QTLs of resistance to mildew identified in grapevine genome by methods of molecular genetics. The number of DNA markers linked to resistance loci are identified. Developments of scientists in this area can be used to study the accumulated national gene pool of grapevines, which will enhance the knowledge of the genetic diversity of the gene pool, identify resistance donors, improve the selection process. Gene Rpv3 is one of the major locus of resistance to mildew, has several haplotypes. North American species such as V. rupestris, V. labrusca and V. riparia are the gene donors. The identification of the gene, according to the published data, is possible by the flanking microsatellite markers UDV305, UDV737. At the indicated microsatellite loci using the automatic genetic analyzer ABI Prism 3130, 23 genotypes of grapes were studied. The analysis showed that in the DNA of the varieties Nadezhda AZOS, Kristall, Kutuzovskyi and Lydia, the resistance gene Rpv3 is present. In cultivars Nadezhda AZOS, Kristall, Kutuzovskyi defined a haplotype Rpv3299-279, Lydia - Rpv3null-287.
Keywords: Grapevines, resistance to downy mildew, resistance gene Rpv3, DNA-markers
DOI: 10.21515/1999-1703-67-63-67


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  1. Ilnitskaya Elena Tarasovna, Cand. Biol. Sci, Head of laboratory, FSBSI "North Caucasian Regional Research Institute of Horticulture and Viticulture".
  2. Tokmakov Sergey Vyacheslavovich, Cand. Biol. Sci, Research Associate, FSBSI "North Caucasian Regional Research Institute of Horticulture and Viticulture".
  3. Makarkina Marina Viktorovna, a junior researche, Post-graduate student, FSBSI "North Caucasian Regional Research Institute of Horticulture and Viticulture".