Труды Кубанского государственного аграрного университета

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2017, № 67

UDC: 57.085.23
GSNTI: 62.33.29

Micropropagation of Albidae Dode section poplar hybrids

Poplar is one of the rapidly growing tree species. Introduction of selected, resistant to unfavorable environmental factors material into forestry practice is limited by low efficiency of vegetative propagation of some poplar species. The application of the in vitro culture method allows to quickly multiply valuable biotypes. In the process of micropropagation of woody plants morphogenetic reaction significantly depends on genotype, age, source material. These factors require optimization of culture conditions and the composition of culture media for specific forms, varieties, hybrids. Poplar micropropagation was carried out by activation of axillary meristems. Using isolated in June young shoots lets to obtain up to 76,3% aseptic viable cultures. The results showed that the highest percentage of morphogenic cultures was obtained using ½ WPM medium, containing BAP 0,2-0,5 mg/l. For elongation the shoots are transplanted to fresh medium every 30-35 days. The shoots 1,5-2 cm long were rooted in the hormone-free media and with the addition of auxin. The maximum rooting (50%) was achieved using IBA 0,01 mg/l as root inducer. The efficiency of all micropropagation stages is significantly depends on the genotype of the source tree. For all test forms were obtained regenerated plants useful for mass micropropagation.
Keywords: Poplar, micropopagation, explant, culture medium, growth regulators
DOI: 10.21515/1999-1703-67-301-304


  1. А. с. на селекционное достижение № 67320 Тополь Ведуга / А. П. Царев, ФГБУ Всероссийский научно-исследовательский институт лесной генетики, селекции и биотехнологии. - По заявке № 8456511, 2016.
  2. А. с. на селекционное достижение № 67322 Тополь Болид / А. П. Царев. - ФГБУ Всероссийский научно-исследовательский институт лесной генетики, селекции и биотехнологии. - По заявке № 8456512, 2016.
  3. Машкина, О. С. Рекомендации по сохранению и воспроизводству методами биотехнологии ценных генотипов карельской березы, осины, тополя белого и сереющего/ О. С. Машкина, Т. М. Табацкая. - Воронеж: НИИЛГиС, 2005. - 29 с.
  4. Царев, А. П. Селекция и репродукция лесных древесных пород / А. П. Царев, С. П. Погиба, В. В. Тренин. - Учебник. - Москва: Логос, 2001. - 520 с.
  5. Bradshaw, H. D. Emerging Model Systems in Plant Biology: Poplar (Populus) as a Model Forest Tree / H. D. Bradshaw // J. Plant. - Growth. Regul. - 2000. - V. 19. - P. 306-313.
  6. Klopfenstein, N. B. Micropropagation, genetic engineering and molecular biology of Populus / N. B. Klopfenstein, Y. W. Chun, M.-S. Kim, M. R. Ahuja. - USDA For. Serv. Gen. Tech. Rep. RM-GTR-297, Rocky Mountain Forest and Range Experiment Station, Fort Collins, CO. - 1997. - 326 p.
  7. Lloyd, G. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use shoot tip culture / G. Lloyd, D. McCown // Plant Ptopagators Soc. - Comb. Proc. - 1980. - P. 421-427.
  8. Murashige, T. A revised medium for rapid growth and boisays with tobacco tissue culture / T. Murashige, F. A. Scoog // Phisiol. Planetarium. - 1962. - V. 15. - № 3. - P. 473-497.
  9. Srivastava, S. Tissue culture studies on chinese poplar (Populus tomentosa) / S. Srivastava, H. Glock and Zh. Zhang. - Silvae Genet. - 1990. - Vol. 40. - P. 247-248.
  10. Zhang, B. Y. 2008. Establishment of highly efficient plant regeneration system of Populus davidiana ½ Populus bolleana and study of its genetic stability / B. Y. Zhang, X. H. Su, S. X. Zheng. - Beijing For Univ. - 30: 68-73.


  1. Shabanova Ekaterina Aleksandrovna, research scientists, All-Russian Research Institute of Forest Genetics, Breeding and Biotechnology.