Труды Кубанского государственного аграрного университета


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2016, № 60

UDC: 57.083.134:577.17:582.929.4
GSNTI: 62.33.29

The influence of hormonal composition of nutrient medium on the induction of callusogenesis and somatic organogenesis of Agastache foeniculum (Pursh) Kuntze (Lamiaceae)

Plant secondary metabolites have wide range of biological activity and can be used in economic activities. Obtaining secondary metabolites through tissue and cells plants cultures is a promising technology. It is possible to carry out a targeted synthesis of secondary metabolites in vitro culture by variation different factors, such as cell line selection, qualitative and quantitative compounds of nutrient medium, addition of elicitors and synthesis precursors, bioreactor design. Hormonal composition of nutrient medium is the most easily regulated factor, which has a significant impact on secondary metabolites accumulation level. Agastache plants have a high level of biological activities. Different compounds obtained from Agastache genus plants have anesthetic, anti-inflammatory, anticancerogenic, antimicrobial and other pharmacological properties. Tissues and cells cultures of Agastache plants can be used for obtaining volatile or non-volatile specialized secondary metabolites such as rosmarinic acid and volatile terpenoid compounds of essential oils (methylchavicol, menthone, pulegone etc.). Rosmarinic acid has high level of antioxidant activity and large potential for treatment Alzheimer disease and chemotherapy of cancer. Essential oils of different Agastache species are widely used in ethnomedicine as well as anti-inflammatory, antibacterial, soothing influence. Effect of hormonal composition in cultivation medium on induction of callusogenesis and somatic organogenesis Agastache foeniculum were evaluated in this study.
Keywords: Secondary metabolism, medicinal herb, Agastache, Lamiaceae, in vitro culture, callusogenesis, somatic organogenesis

References:

  1. Мазур, Т. В. Регуляция ростовых процессов каллусной культутры представителей семейства Lamiaceae / Т. В. Мазур, И. Ф. Вайновская, И. М. Чумакова, Т. И. Фоменко // Интродукция, сохранение и использование биологического разнообразия мировой флоры: материалы международной конференции, посвященной 80-летию Центрального ботанического сада Национальной академии наук Беларуси (19-22 июня 2012, Минск, Беларусь). - 2012. - Ч. 2. - С. 419-423.
  2. Носов, А. М. Регуляция синтеза вторичных соединений в культуре клеток растений / А. М. Носов // Биология культивируемых клеток и биотехнология растений / под ред. Р. Г. Бутенко. - М.: Наука, 1991. - С. 5-20.
  3. Чередниченко, М. Ю. Перспективы биотехнологических методов размножения представителей рода Agastache Clayton ex Gronov. для получения вторичных метаболитов / М. Ю. Чередниченко, О. Б. Поливанова // Труды Кубанского государственного аграрного университета. - 2015. - № 4 (55). - С. 282-286.
  4. Kayani, H. A. Micropropagation of Agastache anisata using nodal segments as explants and cytotoxic activity of its methanolic extracts / H. A. Kayani, S. Khan, S. Naz // Pak J Bot. - 2013. - № 45(6). - P. 2105-2109.
  5. Kim, Y. B. Metabolomics analysis and biosynthesis of rosmarinic acid in Agastache rugosa Kuntze treated with methyl jasmonate / Y. B. Kim, J. K. Kim, M. R. Uddin // PLoS ONE. - 2013. - № 8(5). - е64199.
  6. Mazur, T. V. Cultivation of Agastache rugosa (Fisch. et Mey) Kuntze in vitro. / T. V. Mzur, V. N. Reshetnikov // Becцi Haцыянaльнaй aкaдэмii нaвyк Бeлapyci. Cepыя бiялaгiчныx нaвyк. - 2005. - № 3. - P. 5-9.
  7. Menghini, A. Pigment content and methyl chavicol production in Agastache foeniculum Kuntze cultured in vitro / A. Menghini, M. Capu-ccella, R. Pagiotti // J Essent Oil Res. - 1992. - № 4 (5). - P. 483-486.
  8. Shin, S. Production of volatile oil components by cell culture of Agastache rugosa O. Kuntze / S. Shin, Y. S. Kim, C. A. Kang // Nat Prod Sci. - 2001. - № 77. - P. 120-123.
  9. Wu, J. Production of Ginseng and its bioactive components in plant cell culture: current technological and applied aspect / J. Wu, J.-J. Zhong // J. Biotechnol. - 1999. - Vol. 68. - P. 89-99.
  10. Xu, H. Rosmarinic acid biosynthesis in callus and cell cultures of Agastache rugosa Kuntze / H. Xu, Y. K. Kim, X. Jin // J Med Plants Res. - 2008. - Vol. 2(9). - P. 237-241.
  11. Zielin´ska, S. Influence of plant growth regulators on volatiles produced by in vitro grown shoots of Agastache rugosa (Fisher & C.A.Meyer) O. Kuntze / S, Zielin´ska, E. Piatczak, D. Kalemba // Plant Cell Tissue Organ Cult. - 2001. - № 107. - P. 161-167.

Authors:

  1. Polivanova Oksana Borisovna, PhD student, Timiryazev Russian State Agrarian University.
  2. Cherednichenko Mikhail Yuryevich, PhD in Biotechnology, associate professor, Timiryazev Russian State Agrarian University.