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2019, № 81

UDC: 633.63:575:632.52.577.1
GSNTI: 34.15.25

Molecular screening of Beta vulgaris L. breeding material for presence of powdery mildew resistance gene

Powdery mildew is a wide-spread disease that causes premature dying off of leaves, inhibits plants and leads to sugar content decrease in beet roots. Selection and use of genetically resistant sugar beet forms in crosses is the most priority direction today. In the given work, the results of sugar beet breeding material molecular-genetic study for presence/absence of the Pm dominant gene of resistance to powdery mildew have been elucidated. The results of development and approvement of a specific primer pair effective for identification of the Pm powdery mildew resistance gene in sugar beet breeding material are presented. As a result of the performed PCR-analysis using Pm36 specific primer, 6 sugar beet genotypes with the amplicon of 1000 b.p. in length that is characteristic for the Pm gene have been selected. These samples of sugar beet plants have appeared to be carriers of R-genes or loci inherited linked to genes of resistance to the phytopathogen. Screening of sugar beet breeding materials for resistance to powdery mildew using of molecular-genetic primers can be successfully used in practice. These genotypes can be used as a new starting material with resistance to powdery mildew.
Keywords: Polymerase chain reaction (PCR), primer, gene, powdery mildew, sugar beet
DOI: 10.21515/1999-1703-81-138-141

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Authors:

1. Nalbandyan Arpine Artavadzovna, Phd in Biology, Head of Biochemistry and Molecular Biology laboratory, A. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar.
2. Fedulova Tatyana Petrovna, DSc in Biology, A. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar.