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2016, № 63

UDC: 633.16
GSNTI: 34.35

Influence of Na-salt solutions on barley seed germination,seedlings development and on the activity oftheir main NADP-producing enzymes

The concept of the salt tolerance mechanism, the introduction of the received results into practice, the study of the meaningful agricultural plants and increase of their fruitfulness is of paramount importance to the experimenter and is one of the main problems requiring to be solved in the development of agriculture. The ability to partially retain the germinating ability under the influence of salt solutions of similar concentration, on one hand, and deprivation of this attribute, on the other, is apparently a phenomenon associated with the diversity of seed vitality. Rather, the seeds with low vitality are not able to germinate, and perish in accordingly, and the seeds which have a relatively higher vitality are able to maintain this ability under stressful conditions. The impact of different concentration ofNaCl, Na2SO4,NaHCO3 and Na2CO3 solutions on barley (Hordeumvulgaris) seed germination, seedlings development and on the activity of their main NADP-producing enzymes glucose-6-phophate dehydrogenase (G6PDH, EC 1.1.1.49) and malate dehydrogenase (decarboxilating) (MDHD, EC 1.1.1.40) was studied. The use solutions have a negative effect on germination and development of seedlings and correlated with their concentrations. The efficiency of this impact was very strong in the early stage of seedlings. G6PDH activity was high in the early stage, but DMDH activity was higher as compared with the previous one. Activity of both enzymes was induced in the moderate concentrations of salt solutions. G6PDH/ DMDH activity ratio was decreased depending on development of seedlings and impact of salt solutions.
Keywords: Na-salt solutions, barley seedlings, glucose-6-phophate dehydrogenase, malate dehydrogenase (decarbixilating)
DOI: 10.21515/1999-1703-63-77-82

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Authors:

1. Mirzoyeva Besti Qoshun, doctoral student in biochemistry and biotechnology, Baku State University, Azerbaijan.